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Western blot analysis of lysates from wild type (WT) and Claudin 1 knockdown (KD) Hep G2 cells using [KD Validated] Claudin 1 Rabbit mAb (A25902) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 1s.
Western blot analysis of lysates from A-431 cells using [KD Validated] Claudin 1 Rabbit mAb (A25902) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 1s.
Western blot analysis of various lysates using [KD Validated] Claudin 1 Rabbit mAb (A25902) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] Claudin 1 Rabbit mAb (A25902) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] Claudin 1 Rabbit mAb (A25902) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KD Validated] Claudin 1 Rabbit mAb (A25902) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using[KD Validated] Claudin 1 Rabbit mAb (A25902) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using [KD Validated] Claudin 1 Rabbit mAb (A25902) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using [KD Validated] Claudin 1 Rabbit mAb (A25902) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KD Validated] Claudin 1 Rabbit mAb (A25902) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Human colon cancer tissue using [KD Validated] Claudin 1 Rabbit mAb (A25902, dilution 1:50) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Human skin tissue using [KD Validated] Claudin 1 Rabbit mAb (A25902, dilution 1:50) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
Western blot analysis of lysates from wild type (WT) and Claudin 1 knockdown (KD) Hep G2 cells using [KD Validated] Claudin 1 Rabbit mAb (A25902) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 1s.

[KD Validated] Claudin 1 Rabbit Recombinant Antibody

CATALOG NUMBER: 83-181

Clonality:
Monoclonal
Tested Applications:
ELISA, IF-P, IHC-P, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF-P, IHC-P, IP, WB
Application Note:
WB,1:1000 - 1:5000, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, IF-P,1:50 - 1:200, IHC-P,1:50 - 1:200, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
23kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CLDN1
Additional Names:
CLD1; SEMP1; ILVASC
Protein Accession Number:
O95832
Ncbi Gene Id Number:
9076
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-181

List Size:
100 uL

List Price:

$540.00

Datasheet
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