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Western blot analysis of lysates from A-549 cells, using [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 60s.
Western blot analysis of lysates from wild type(WT) and Integrin alpha 5 (ITGA5/CD49e) knockdown (KD) HeLa cells, using [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 60s.
Confocal imaging of U-937 cells using [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706, dilution 1:500) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Western blot analysis of lysates from A-549 cells, using [KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb (A22706) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 60s.

[KD Validated] Integrin alpha 5 (ITGA5/CD49e) Rabbit Recombinant Antibody

CATALOG NUMBER: 82-348

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, WB
Application Note:
WB,1:500 - 1:1000, IF/ICC,1:200 - 1:800, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
HeLa,A-549
Predicted Molecular Weight:
115kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ITGA5
Additional Names:
FNRA; CD49e; VLA-5; VLA5A; [KD Validated] Integrin alpha 5 (ITGA5/CD49e)
Protein Accession Number:
P08648
Ncbi Gene Id Number:
3678
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-348

List Size:
100 uL

List Price:

$540.00

Datasheet
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