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Western blot analysis of various lysates, using [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 180s.
Western blot analysis of lysates from wild type(WT) and KMT5A/SETD8 knockdown (KD) 293T cells, using [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Enhanced Kit (RM00021). | Exposure time: 30s.
Western blot analysis of various lysates, using [KD Validated] KMT5A/SETD8 Rabbit mAb (A4136) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 180s.

[KD Validated] KMT5A/SETD8 Rabbit Recombinant Antibody

CATALOG NUMBER: 83-834

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
293T,HeLa,U-87MG,Mouse brain,Mouse heart
Predicted Molecular Weight:
43kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
KMT5A
Additional Names:
SET8; SET07; SETD8; PR-Set7; PR/SET07; [KO/KD Validated] KMT5A/SETD8
Protein Accession Number:
Q9NQR1
Ncbi Gene Id Number:
387893
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a protein-lysine N-methyltransferase that can monomethylate Lys-20 of histone H4 to effect transcriptional repression of some genes. The encoded protein is required for cell proliferation and plays a role in chromatin condensation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-834

List Size:
100 uL

List Price:

$540.00

Datasheet
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