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Western blot analysis of lysates from wild type (WT) and NNMT knockdown (KD) HeLa cells using [KD Validated] NNMT Rabbit mAb (A25391) at 1:3000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 30s.
Confocal imaging of A549 cells using [KD Validated] NNMT Rabbit mAb (A25391, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunoprecipitation of NNMT in 200 µg extracts from A549 cells using 0.5 µg [KD Validated] NNMT Rabbit mAb (A25391). Western blot analysis was performed using [KD Validated] NNMT Rabbit mAb (A25391) at 1:3000 dilution.
Western blot analysis of lysates from wild type (WT) and NNMT knockdown (KD) HeLa cells using [KD Validated] NNMT Rabbit mAb (A25391) at 1:3000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 30s.

[KD Validated] NNMT Rabbit Recombinant Antibody

CATALOG NUMBER: 83-084

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, IP, WB
Application Note:
WB,1:3000 - 1:12000, IF/ICC,1:200 - 1:800, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
30kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NNMT
Additional Names:
Nicotinamide N-methyltransferase
Protein Accession Number:
P40261
Ncbi Gene Id Number:
4837
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-084

List Size:
100 uL

List Price:

$540.00

Datasheet
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