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Western blot analysis of lysates from RAW 264.7 cells using [KD Validated] TFEB Rabbit mAb (A25981) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 45s.
Western blot analysis of lysates from wild type (WT) and TFEB knockdown (KD) 293T cells using [KD Validated] TFEB Rabbit mAb (A25981) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KD Validated] TFEB Rabbit mAb (A25981) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KD Validated] TFEB Rabbit mAb (A25981) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from Raji cells treated by Torin-1 (250nM, 5h), using 5 μg of [KD Validated] TFEB Rabbit mAb (A25981) and Rabbit IgG isotype control (AC042). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.
Western blot analysis of lysates from RAW 264.7 cells using [KD Validated] TFEB Rabbit mAb (A25981) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 45s.

[KD Validated] TFEB Rabbit Recombinant Antibody

CATALOG NUMBER: 83-197

Clonality:
Monoclonal
Tested Applications:
ChIP, ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ChIP, ELISA, IHC-P, WB
Application Note:
WB,1:1000 - 1:2000, IHC-P,1:200 - 1:800, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements., ChIP,5μg antibody for 10μg-15μg of Chromatin
Predicted Molecular Weight:
44kDa/53kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TFEB
Additional Names:
TCFEB; BHLHE35; ALPHATFEB
Protein Accession Number:
P19484
Ncbi Gene Id Number:
7942
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enables DNA-binding transcription factor activity; enzyme binding activity; and transcription cis-regulatory region binding activity. Involved in several processes, including cellular response to amino acid starvation; lysosome localization; and positive regulation of autophagy. Located in cytosol; lysosomal membrane; and nucleoplasm.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-197

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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