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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with TIFAB using [KD Validated] TIFAB Rabbit mAb (A24024) at 1:2000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 10s. | <b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b>
Western blot analysis of lysates from wild type (WT) and TIFAB knockdown (KD) MV-4-11 cells using [KD Validated] TIFAB Rabbit mAb (A24024) at 1:2000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 30s. | <b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b>
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with TIFAB using [KD Validated] TIFAB Rabbit mAb (A24024) at 1:2000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 10s. | WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

[KD Validated] TIFAB Rabbit Recombinant Antibody

CATALOG NUMBER: 82-848

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:1000 - 1:5000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
293T transfected with TIFAB（Human）, MV-4-11
Predicted Molecular Weight:
18KDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TIFAB
Protein Accession Number:
Q6ZNK6
Ncbi Gene Id Number:
497189
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TIFAB binds to TIFA (MIM 609028) and inhibits TIFA mediated NF kappa-B (NFKB1; MIM 164011) activation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-848

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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