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Western blot analysis of lysates from wild type (WT) and UQCRC1 knockdown (KD) HeLa cells using [KD Validated] UQCRC1 Rabbit mAb (A26343) at 1:5000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 10s.
Western blot analysis of various lysates using [KD Validated] UQCRC1 Rabbit mAb (A26343) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using [KD Validated] UQCRC1 Rabbit mAb (A26343) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KD Validated] UQCRC1 Rabbit mAb (A26343) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] UQCRC1 Rabbit mAb (A26343) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Confocal imaging of HeLa cells using [KD Validated] UQCRC1 Rabbit mAb (A26343, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunoprecipitation of UQCRC1 from 600 µg extracts of Mouse liver tissue was performed using 1 µg of [KD Validated] UQCRC1 Rabbit mAb (A26343). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using [KD Validated] UQCRC1 Rabbit mAb (A26343) at a dilution of 1:1000.
Western blot analysis of lysates from wild type (WT) and UQCRC1 knockdown (KD) HeLa cells using [KD Validated] UQCRC1 Rabbit mAb (A26343) at 1:5000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 10s.

[KD Validated] UQCRC1 Rabbit Recombinant Antibody

CATALOG NUMBER: 83-295

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, IHC-P, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, IHC-P, IP, WB
Application Note:
WB,1:2000 - 1:12000, IHC-P,1:50 - 1:200, IF/ICC,1:50 - 1:200, IP,0.5μg-4μg antibody for 400μg-800μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
53kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
UQCRC1
Additional Names:
QCR1; PKNPY; UQCR1; D3S3191
Protein Accession Number:
P31930
Ncbi Gene Id Number:
7384
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enables ubiquitin protein ligase binding activity. Predicted to be involved in oxidative phosphorylation. Predicted to act upstream of or within mitochondrial electron transport, ubiquinol to cytochrome c. Located in mitochondrion. Implicated in Alzheimer's disease. Biomarker of Alzheimer's disease.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-295

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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