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Western blot analysis of KDM1B in K562 cell lysate with KDM1B antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide
Western blot analysis of KDM1B in K562 cell lysate with KDM1B antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide
Trial Size

KDM1B Antibody

CATALOG NUMBER: 7535

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
KDM1B antibody was raised against a 17 amino acid peptide near the amino terminus of human KDM1B.
The immunogen is located within amino acids 80 - 130 of KDM1B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
KDM1B antibody can be used for detection of KDM1B by Western blot at 0.5 μg/ml.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
KDM1B antibody is human, mouse and rat reactive. At least two isoforms of KDM1B are known to exist; this KDM1B antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Predicted Molecular Weight:
Predicted: 90 kDa
Observed: 90 kDa
Purification:
KDM1B antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
KDM1B antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
KDM1B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
KDM1B
Additional Names:
KDM1B Antibody: AOF1, LSD2, C6orf193, bA204B7.3, dJ298J15.2, AOF1, Lysine-specific histone demethylase 1B, Flavin-containing amine oxidase domain-containing protein 1
Protein Accession Number:
NP_694587
Protein Gi Number:
116256451
Ncbi Gene Id Number:
221656
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Flavin-dependent histone demethylases regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (reviewed in 1). KDM1B is a recently identified histone H3K4 demethylase that is required to establish maternal genomic imprints; targeted disruption of the KDM1B gene in mice led to lethality in their embryos, suggesting demethylation of H3K4 is critical for establishing the DNA methylation imprints (2). KDM1B has also been shown to play a role in the transcription elongation in several genes (3).
Background Reference 1:
Culhane JC and Cole PA. LSD1 and the chemistry of histone demethylation. Curr. Opin. Chem. Biol. 2007; 11:561-8.
Background Reference 2:
Ciccone DN, Su H, Hevi S, et al. KDM1B is a histone H3K4 demethylase required to establish maternal genomic imprints. Nature 2009; 461:415-8.
Background Reference 3:
Fang R, Barbera A, Xu Y, et al. Human LSD2/KDM1b/AOF1 regulates genes transcription by modulating intragenic H3K4me2 methylation. Mol. Cell 2010; 39:222-233.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7535

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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