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Western blot analysis of extracts of various cell lines, using KDM4A antibody (23-306) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using KDM4A antibody (23-306) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using KDM4A antibody (23-306) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using KDM4A antibody (23-306) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using KDM4A antibody (23-306) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

KDM4A Antibody

CATALOG NUMBER: 23-306

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 585-815 of human KDM4A (NP_055478.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
IP: 1:50 - 1:100
Positive Control 1:
BT-474
Positive Control 2:
THP-1
Positive Control 3:
Mouse thymus
Predicted Molecular Weight:
Observed: 150kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
KDM4A
Additional Names:
JMJD2A Antibody: JMJD2, JHDM3A, JMJD2A, TDRD14A, JMJD2, KIAA0677, Lysine-specific demethylase 4A, JmjC domain-containing histone demethylation protein 3A
Ncbi Gene Id Number:
9682
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-306

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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