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Western blot analysis of lysates from T-47D cells using KDM4B/JMJD2B Rabbit mAb at 1:7000 dilution incubated at room temperature for 1.5 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5 s.
Immunofluorescence analysis of C6 cells using KDM4B/JMJD2B Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using KDM4B/JMJD2B Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of HCT 116 cells using 3 μg KDM4B/JMJD2B Rabbit mAb. Western blot was performed from the immunoprecipitate using KDM4B/JMJD2B Rabbit mAb at a dilution of 1:1000.
Chromatin immunoprecipitation analysis of extracts of 5637 cells, using KDM4B/JMJD2B Rabbit mAb and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Western blot analysis of lysates from T-47D cells using KDM4B/JMJD2B Rabbit mAb at 1:7000 dilution incubated at room temperature for 1.5 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5 s.

KDM4B/JMJD2B Rabbit Recombinant Antibody (84-175)

CATALOG NUMBER: 84-175

Clonality:
Monoclonal
Tested Applications:
ChIP, ELISA, IF/ICC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ChIP, ELISA, IF/ICC, IP, WB
Application Note:
WB,1:500 - 1:2000, IF/ICC,1:50 - 1:200, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements., ChIP,5μg antibody for 10μg-15μg of Chromatin
Positive Control 1:
HCT 116,Mouse brain
Predicted Molecular Weight:
122kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
KDM4B
Additional Names:
MRD65; JMJD2B; TDRD14B; KDM4B/JMJD2B
Protein Accession Number:
O94953
Ncbi Gene Id Number:
23030
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enables histone H3-methyl-lysine-36 demethylase activity and histone H3-methyl-lysine-9 demethylase activity. Involved in histone H3-K36 demethylation and histone H3-K9 demethylation. Located in cytosol and nucleoplasm. Implicated in autosomal dominant non-syndromic intellectual disability; breast cancer; colorectal cancer; malignant peripheral nerve sheath tumor; and stomach cancer. Biomarker of several diseases, including alopecia areata; lung cancer; medulloblastoma; prostate cancer; and stomach cancer.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-175

List Size:
100 uL

List Price:

$540.00

Datasheet
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