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Western blot analysis of various lysates using KDM5A Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 3min.
Western blot analysis of lysates from Mouse lung, using KDM5A Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit. | Exposure time: 3min.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg KDM5A antibody. Western blot was performed from the immunoprecipitate using KDM5A antibody at a dilution of 1:500.
Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using KDM5A antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Western blot analysis of various lysates using KDM5A Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 3min.

KDM5A Rabbit Recombinant Antibody (83-998)

CATALOG NUMBER: 83-998

Clonality:
Monoclonal
Tested Applications:
ChIP, ELISA, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ChIP, ELISA, IP, WB
Application Note:
WB,1:500 - 1:2000, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ChIP,2μg antibody for 10μg-15μg of Chromatin, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
HeLa,HepG2,Mouse lung
Predicted Molecular Weight:
192kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
KDM5A
Additional Names:
RBP2; RBBP2; RBBP-2; KDM5A
Protein Accession Number:
P29375
Ncbi Gene Id Number:
5927
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-998

List Size:
100 uL

List Price:

$540.00

Datasheet
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