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Western blot analysis of KDR (Y1175) Antibody in mouse lung tissue lysates (35ug/lane)
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of KDR (Y1175) Antibody in mouse lung tissue lysates (35ug/lane)

KDR Antibody

CATALOG NUMBER: 63-093

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This KDR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1153-1182 amino acids from human KDR.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
152 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KDR
Additional Names:
Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, FLK-1, Kinase insert domain receptor, KDR, Protein-tyrosine kinase receptor flk-1, CD309, KDR, FLK1, VEGFR2
Protein Accession Number:
P35968
Protein Gi Number:
9087218
Ncbi Gene Id Number:
3791
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KDR is a major growth factor for endothelial cells. This protein encodes one of the two receptors of the KDR. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc..
Background Reference 1:
Huang, D., et al. Cancer Res. 70(3):1053-1062(2010)
Background Reference 2:
Sentilhes, L., et al. J. Neuropathol. Exp. Neurol. 69(2):111-128(2010)
Background Reference 3:
Lamalice, L., et al. J. Biol. Chem. 281(45):34009-34020(2006)
Background Reference 4:
Meyer, R.D., et al. J. Biol. Chem. 277(30):27081-27087(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-093

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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