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42-391 (0.2ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer).. Detected by chemiluminescence.
42-391 (3.8ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-391 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear st
42-391 (0.2ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer).. Detected by chemiluminescence.

KEAP1 Antibody

CATALOG NUMBER: 42-391

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-EVTPSQHGNRTFS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 69.7kDa according to Human NP_036421.2 and of 69.6kDa according to Mouse NP_057888.1). Recommended concentration: 0.2-0.6ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebellum shows textured cytoplasm staining in Purkinje cell and processes. Recommended concentration, 3-5ug/ml.Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells. Recommended concentration: 5ug/ml.
Specificity:
Reported variants represent identical protein: NP_036421.2, NP_987096.1
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
KEAP1
Additional Names:
KEAP1, kelch-like ECH-associated protein 1, INrf2, KIAA0132, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, MGC9454, cytosolic inhibitor of Nrf2, kelch-like protein 19
Protein Accession Number:
NP_036421.2
Protein Gi Number:
22027642
Ncbi Gene Id Number:
9817

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-391

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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