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IHC testing of FFPE human skin with Keratin 10 antibody (clone KRT10/844). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
IHC testing of FFPE human skin with Keratin 10 antibody (clone KRT10/844). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
IHC testing of FFPE human bladder carcinoma with Keratin 10 antibody (clone KRT10/844). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free Keratin 10 Antibody (clone KRT10/844). Confirmation of Integrity and Purity of the Antibody.
IHC testing of FFPE human skin with Keratin 10 antibody (clone KRT10/844). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.

Keratin 10 Antibody [KRT10/844]

CATALOG NUMBER: 33-429

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant human KRT10 protein was used as the immunogen for the Keratin 10 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.1-0.2 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Optimal dilution of the Keratin 10 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
KRT10
Additional Names:
Keratin, type I cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KRT10, KPP
Ncbi Gene Id Number:
3858
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This mAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-429

List Size:
100 ug

List Price:

$626.00

Datasheet
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