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Western blot analysis of lysed extracts from HT29 cells untreated or treated with EGF using Keratin 18 (Phospho-Ser33).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 18 (Phospho-Ser33).
Western blot analysis of lysed extracts from HT29 cells untreated or treated with EGF using Keratin 18 (Phospho-Ser33).

Keratin 18 (phospho Ser33) Antibody

CATALOG NUMBER: 79-233

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Keratin 18 (Phospho-Ser33) antibody was raised against a peptide sequence around phosphorylation site of serine 33 (A-A-S (p) -V-Y) derived from Human Keratin 18 (CK18).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of Keratin 18 only when phosphorylated at serine 33.
Predicted Molecular Weight:
46 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
KRT18
Additional Names:
K18, CYK18, CBL2, RNF55, CK 18, CK18, Cytokeratin endo B, K18
Protein Accession Number:
NP_000215.1
Protein Gi Number:
4557888
Ncbi Gene Id Number:
3875
Background:
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. utations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Background Reference 1:
Ramaekers, F. C. and Bosman, F. T. (2004) J. Pathol. 204, 351-354. Chang, L. and Goldman, R. D. (2004) Nat. Rev. Mol. Cell Biol. 5, 601-613. Moll, R. et al. (1982) Cell 31, 11-24.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-233

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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