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Western blot analysis of lysed extracts from HUVEC and 293 cells using Keratin 8 (Ab-74).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 8 (Ab-74).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using Keratin 8 (Ab-74).
Western blot analysis of lysed extracts from HUVEC and 293 cells using Keratin 8 (Ab-74).

Keratin 8 (Ab 74) Antibody

CATALOG NUMBER: 79-410

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Keratin 8 (Ab-74) antibody was raised against a peptide sequence around aa.72~76 (L-L-S-P-L) derived from Human Keratin 8 (CK8).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total Keratin 8 protein.
Predicted Molecular Weight:
55 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
KRT8
Additional Names:
K8, KO, CK8, CK-8, CYK8, K2C8, CARD2, HSTF1, CK 8, Cytokeratin endo A, K8
Protein Accession Number:
NP_002264.1
Protein Gi Number:
4504919
Ncbi Gene Id Number:
3856
Background:
Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
Background Reference 1:
Nakamichi I, et al. (2002) Mol Biol Cell; 13 (10): 3441-3451. Prochasson P, et al. (2002) Nucleic Acids Res; 30 (15): 3312-3322. Pankov R, et al. (1994) Proc Natl Acad Sci U S A; 91 (3): 873-877. Thorey IS, et al. (1993) Mol Cell Biol; 13 (11): 6742-6751.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-410

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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