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Western blot analysis of KHK using rabbit polyclonal Ketohexokinase (KHK) Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KHK gene (Lane 2).
Antibody is used in Western blot to detect KHK in mouse liver tissue lysate (Lane 1) and 293 cell lysate (Lane 2).
Western blot analysis of KHK using rabbit polyclonal Ketohexokinase (KHK) Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KHK gene (Lane 2).

Ketohexokinase Antibody

CATALOG NUMBER: 62-632

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This Ketohexokinase (KHK) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-46 amino acids from the N-terminal region of human Ketohexokinase (KHK).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KHK
Additional Names:
Ketohexokinase, Hepatic fructokinase, KHK
Protein Accession Number:
P50053
Protein Gi Number:
1730044
Ncbi Gene Id Number:
3795
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ketohexokinase (KHK), or fructokinase, catalyzes conversion of fructose to fructose-1-phosphate. Splice variant 1 is the highly active form found in liver, renal cortex, and small intestine, while splice variant 2 is the lower activity form found in most other tissues. KHK, like glucokinase (GCK) and glucokinase regulator (GCKR), is present in both liver and pancreatic islets. The inhibition of GCK by GCKR is blocked by binding of fructose-1-phosphate to GCKR. The chromosomal proximity of the metabolically connected GCKR and KHK genes has a genetic linkage in type 2 diabetes. Fructosuria, or hepatic fructokinase deficiency, is a benign, asymptomatic defect of intermediary metabolism associated with heterozygosity for G50R and A43T mutations in KHK.
Background Reference 1:
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Background Reference 2:
Hayward, B.E., et al., Eur. J. Biochem. 257(1):85-91 (1998).
Background Reference 3:
Bonthron, D.T., et al., Hum. Mol. Genet. 3(9):1627-1631 (1994).

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-632

List Size:
400 ul

List Price:

$495.00

Datasheet
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