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Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
KHSRP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of KHSRP Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

KHSRP Antibody

CATALOG NUMBER: 56-004

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This KHSRP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 470-498 amino acids from the Central region of human KHSRP.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
73 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KHSRP
Additional Names:
Far upstream element-binding protein 2, FUSE-binding protein 2, KH type-splicing regulatory protein, KSRP, p75, KHSRP, FUBP2
Protein Accession Number:
Q92945
Protein Gi Number:
313104306
Ncbi Gene Id Number:
8570
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The KHSRP gene encodes a multifunctional RNA-bindingprotein implicated in a variety of cellular processes, includingtranscription, alternative pre-mRNA splicing, and mRNA localization(Min et al., 1997 [PubMed 9136930]; Gherzi et al., 2004 [PubMed15175153]).
Background Reference 1:
Michlewski, G., et al. Nat. Struct. Mol. Biol. 17(8):1011-1018(2010)
Background Reference 2:
Bikkavilli, R.K., et al. J. Cell. Sci. 123 (PT 8), 1352-1362 (2010) :
Background Reference 3:
Malz, M., et al. Hepatology 50(4):1130-1139(2009)
Background Reference 4:
Trabucchi, M., et al. Nature 459(7249):1010-1014(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-004

List Size:
400 ul

List Price:

$495.00

Datasheet
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