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Western Blot at 1:1000 dilution Lane 1: LNCaP whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human brain with KHSRP Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Immunofluorescence analysis of KHSRP Antibody with paraffin-embedded human brain tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
Western Blot at 1:1000 dilution Lane 1: LNCaP whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

KHSRP Antibody

CATALOG NUMBER: 62-510

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken
Immunogen:
This KHSRP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-122 amino acids from the N-terminal region of human KHSRP.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
73 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KHSRP
Additional Names:
Far upstream element-binding protein 2, FUSE-binding protein 2, KH type-splicing regulatory protein, KSRP, p75, KHSRP, FUBP2
Protein Accession Number:
Q92945
Protein Gi Number:
313104306
Ncbi Gene Id Number:
8570
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KHSRP binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). It is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. It may interact with single-stranded DNA from the far-upstream element (FUSE) and activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
Background Reference 1:
Nechama,M., et.al., FASEB J. 22 (10), 3458-3468 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-510

List Size:
400 ul

List Price:

$495.00

Datasheet
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