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Western blot analysis of extracts of NIH/3T3 cells, using Ki67 antibody (16-798) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of C6 cells using Ki67 antibody (16-798) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using Ki67 antibody (16-798) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of NIH/3T3 cells, using Ki67 antibody (16-798) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Ki67 Antibody

CATALOG NUMBER: 16-798

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human Ki67
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
NIH/3T3
Predicted Molecular Weight:
Observed: 358kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MKI67
Additional Names:
monoclonal Ki-67, Proliferation-related Ki-67 antigen, Ki-67, KIA, HGNC:7107, antigen identified by monoclonal Ki-67, MKI67, MIB-1
Ncbi Gene Id Number:
4288
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-798

List Size:
100 uL

List Price:

$436.00

Datasheet
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