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Western blot analysis of extracts of HepG2 cells, using Ki67 antibody (13-879).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse spleen using Ki67 antibody (13-879) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using Ki67 antibody (13-879) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human spleen using Ki67 antibody (13-879) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using Ki67 antibody (13-879) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A431 cells using Ki67 antibody (13-879).
Western blot analysis of extracts of HepG2 cells, using Ki67 antibody (13-879).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Ki67 Antibody [E0F7]

CATALOG NUMBER: 13-879

Clonality:
Monoclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein of human Ki67
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HepG2
Predicted Molecular Weight:
Observed: 359kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MKI67
Additional Names:
monoclonal Ki-67, Proliferation-related Ki-67 antigen, Ki-67, KIA, HGNC:7107, antigen identified by monoclonal Ki-67, MKI67, MIB-1
Ncbi Gene Id Number:
4288
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-879

List Size:
50 uL

List Price:

$350.00

Datasheet
Shipping Info

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Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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