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Western blot analysis of extracts of various cell lines, using KIF20B antibody (16-013) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using KIF20B antibody (16-013) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

KIF20B Antibody

CATALOG NUMBER: 16-013

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1500 of human KIF20B (NP_057279.2).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:200 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
U-251MG
Positive Control 2:
OVCAR3
Positive Control 3:
293T
Positive Control 4:
HT-29
Positive Control 5:
NIH/3T3
Positive Control 6:
Mouse brain
Predicted Molecular Weight:
Observed: 210kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
KIF20B
Additional Names:
CT9KRMP1, MPHOSPH1, MPP-1, MPP1, kinesin-like protein KIF20B, M-phase phosphoprotein 1, cancer/testis antigen 9kinesin-related motor interacting with PIN1, mitotic kinesin-like protein, mitotic kinesin-related protein
Ncbi Gene Id Number:
9585
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-013

List Size:
100 uL

List Price:

$436.00

Datasheet
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