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43-440 (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-440 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-440 (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

kininogen 1 Antibody

CATALOG NUMBER: 43-440

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-RITEATKTVGSDT
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 80+60+30kDa bands observed in Human Kidney lysates (calculated MW of 72.0kDa according to NP_001095886.1). The observed MW bands are explained by glycosylation and the splitting into heavy and light chains from the precursor protein. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_001095886.1; NP_000884.1; NP_001159923.1).
Predicted Molecular Weight:
Approx 80 + 60 + 30kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
kininogen 1
Additional Names:
KNG1, kininogen 1, BDK, KNG, HMWK, alpha-2-thiol proteinase inhibitor, bradykinin, fitzgerald factor, high molecular weight kininogen, kininogen-1, williams-Fitzgerald-Flaujeac factor
Protein Accession Number:
NP_001095886.1,NP_000884.1,NP_001159923.1
Protein Gi Number:
156231037
Ncbi Gene Id Number:
3827
Background Reference 1:
Kolte D, Osman N, Yang J, Shariat-Madar Z.High molecular weight kininogen activates B2 receptor signaling pathway in human vascular endothelial cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-440

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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