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Western blot analysis of KIR3DL3 Antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human tonsil reacted with KIR3DL3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit se was used for the analysis.
Western blot analysis of KIR3DL3 Antibody in K562 cell line lysates (35ug/lane)

KIR3DL3 Antibody

CATALOG NUMBER: 63-818

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This KIR3DL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 132-158 amino acids from the Central region of human KIR3DL3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KIR3DL3
Additional Names:
Killer cell immunoglobulin-like receptor 3DL3, CD158 antigen-like family member Z, Killer cell inhibitory receptor 1, CD158z, KIR3DL3, CD158Z, KIR3DL7, KIRC1
Protein Accession Number:
Q8N743
Protein Gi Number:
269849611
Ncbi Gene Id Number:
115653
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KIR3DL3 is transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. This protein are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC).
Background Reference 1:
Trompeter,H.I., et.al., J. Immunol. 174 (7), 4135-4143 (2005)
Background Reference 2:
Arnheim,L., et.al., Tissue Antigens 65 (3), 252-259 (2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-818

List Size:
400 ul

List Price:

$495.00

Datasheet
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