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Antibody used in WB on HepG2 at 0.2-1 ug/ml.
Antibody used in WB on HepG2 at 0.2-1 ug/ml.

KIRREL Antibody

CATALOG NUMBER: 26-225

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KIRREL.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
KIRREL antibody can be used for detection of KIRREL by ELISA at 1:312500. KIRREL antibody can be used for detection of KIRREL by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
66 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store KIRREL antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
KIRREL
Additional Names:
KIRREL, FLJ10845, MGC129542, MGC129543, NEPH1
Protein Accession Number:
NP_060710
Protein Gi Number:
166295173
Ncbi Gene Id Number:
55243
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KIRREL (NEPH1) is a member of the nephrin-like protein family, which includes NEPH2 and NEPH3. The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration. NEPH1 is a member of the nephrin-like protein family, which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2; MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]).
Background Reference 1:
Harita, Y., (2008) J. Biol. Chem. 283 (14), 9177-9186.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-225

List Size:
100 ul

List Price:

$519.00

Datasheet
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