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Western blot analysis of KIRREL3 in mouse kidney tissue lysate with KIRREL3 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of KIRREL3 in mouse kidney tissue with KIRREL3 antibody at 5 μg/ml.
Immunofluorescence of KIRREL3 in mouse kidney tissue with KIRREL3 antibody at 20 μg/ml.
Western blot analysis of KIRREL3 in mouse kidney tissue lysate with KIRREL3 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

KIRREL3 Antibody

CATALOG NUMBER: 7973

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
KIRREL3 antibody was raised against a 19 amino acid peptide near the amino terminus of human KIRREL3.
The immunogen is located within amino acids 20 - 70 of KIRREL3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
KIRREL3 antibody can be used for detection of KIRREL3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
KIRREL3 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. KIRREL3 antibody is predicted to not cross-react with other members of the KIRREL protein family.
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Predicted Molecular Weight:
Predicted: 64, 84 kDa
Observed: 60 kDa
Purification:
KIRREL3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
KIRREL3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
KIRREL3 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KIRREL3
Additional Names:
Kin of irregular chiasm-like protein 3, Kin of IRRE-like protein 3, MRD4, KIRRE, NEPH2, PRO4502, Nephrin-like protein 2
Protein Accession Number:
NP_115920
Protein Gi Number:
26006461
Ncbi Gene Id Number:
84623
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KIRREL3, also known as nephrin-like protein 2, is a type I transmembrane protein belonging to a family of three podocin interacting proteins and the immunoglobulin superfamily (1). KIRREL3 is involved in the regulation of both glomerular and neural development (2), and more specifically, the nucleogenesis of the pontine nuclei in the developing hindbrain (3). KIRREL3 has also been shown to interact with the synaptic scaffold protein calmodulin-associated serine/threonine kinase (CASK) in neuronal cells (4).
Background Reference 1:
Sellin L, Huber TB, Gerke P, et al. NEPH1 defines a novel family of podocin interacting proteins. FASEB J. 2003; 17:115-7.
Background Reference 2:
Neumann-Haefelin E, Kramer-Zucker A, Slanchev K, et al. A model organism approach: defining the role of Neph proteins as regulators of neuron and kidney morphogenesis. Hum. Mol. Genet. 2010; 19:2347-59.
Background Reference 3:
Nishida K, Nakayama K, Yoshimura S, et al. Role of Neph2 in pontine nuclei formation in the developing hindbrain. Mol. Cell Neurosci. 2011; 46:662-70.
Background Reference 4:
Mizuhara E, Minaki Y, Nakatani T, et al. Purkinje cells originate from cerebellar ventricular zone progenitors positive for Neph3 and E-cadherin. Dev. Biol. 2010; 338:202-14.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7973

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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