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Western Blot at 1:1000 dilution Lane 1: human skeletal muscle lysate Lane 2: A-673 whole cell lysate Lysates/proteins at 20 ug per lane.
Antibody is used in Western blot to detect KIST in K562 cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2).
Western Blot at 1:1000 dilution Lane 1: human skeletal muscle lysate Lane 2: A-673 whole cell lysate Lysates/proteins at 20 ug per lane.

KIST Antibody

CATALOG NUMBER: 62-628

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This KIST (KIS) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-295 amino acids from the C-terminal region of human KIST (KIS).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
UHMK1
Additional Names:
Serine/threonine-protein kinase Kist, Kinase interacting with stathmin, PAM COOH-terminal interactor protein 2, P-CIP2, U2AF homology motif kinase 1, UHMK1, KIS, KIST
Protein Accession Number:
Q8TAS1
Protein Gi Number:
24211880
Ncbi Gene Id Number:
127933
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KIST, a member of the Ser/Thr protein kinase family, is a pyruvate kinase that catalyzes formation of phosphoenolpyruvate from pyruvate and ATP. A role for the primarily nuclear KIST protein in mediation of cellular metabolism has been postulated based on the interaction identified with thyroid hormone. KIST is widely expressed, with highest abundance in skeletal muscle, kidney, placenta and peripheral blood leukocytes. Upon serum stimulation, KIST phosphorylates CDKN1B/p27Kip1, thereby regulating the subcellular location of CDKN1B and cell cycle progression in the G1 phase. KIST, which contains one RNA recognition motif domain, has been proposed to partipate in trafficking and processing of RNA. KIST binds to Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells.
Background Reference 1:
Bieche, I., et al., Brain Res. Mol. Brain Res. 114(1):55-64 (2003).
Background Reference 2:
Boehm, M., et al., EMBO J. 21(13):3390-3401 (2002).
Background Reference 3:
Caldwell, B.D., et al., J. Biol. Chem. 274(49):34646-34656 (1999).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-628

List Size:
400 ul

List Price:

$495.00

Datasheet
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