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Western blot analysis of KLC2 Antibody in mouse NIH-3T3 cell line lysates (35ug/lane)
KLC2 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of KLC2 Antibody in mouse NIH-3T3 cell line lysates (35ug/lane)

KLC2 Antibody

CATALOG NUMBER: 64-123

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This KLC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 403-432 amino acids from the C-terminal region of human KLC2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
69 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KLC2
Additional Names:
Kinesin light chain 2, KLC 2, KLC2
Protein Accession Number:
Q9H0B6
Protein Gi Number:
13878553
Ncbi Gene Id Number:
64837
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Kinesin is a molecular motor that generates ATP-dependent movement of vesicles and organelles along microtubules. Kinesin consists of 2 light chains, such as KLC2, and 2 heavy chains (see KIF5B; MIM 602809) in a 1:1 stoichiometric ratio.
Background Reference 1:
Trejo, H.E., et al. FASEB J. 24(2):374-382(2010)
Background Reference 2:
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Background Reference 3:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Background Reference 4:
Olsen, J.V., et al. Cell 127(3):635-648(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-123

List Size:
400 ul

List Price:

$495.00

Datasheet
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