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Antibody used in WB on Human 721_B cells at 0.2-1 ug/ml.
Antibody used in WB on Human 721_B cells at 0.2-1 ug/ml.

KLF12 Antibody

CATALOG NUMBER: 25-462

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KLF12.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
KLF12 antibody can be used for detection of KLF12 by ELISA at 1:1562500. KLF12 antibody can be used for detection of KLF12 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
721_B Cell Lysate
Predicted Molecular Weight:
44 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store KLF12 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
KLF12
Additional Names:
KLF12, AP-2rep, AP2REP, HSPC122
Protein Accession Number:
NP_009180
Protein Gi Number:
21071074
Ncbi Gene Id Number:
11278
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Activator protein-2 alpha (AP-2 alpha) is a developmentally-regulated transcription factor and important regulator of gene expression during vertebrate development and carcinogenesis. KLF12 is a member of the Kruppel-like zinc finger protein family and can repress expression of the AP-2 alpha gene by binding to a specific site in the AP-2 alpha gene promoter. Repression by the encoded protein requires binding with a corepressor, CtBP1.Activator protein-2 alpha (AP-2 alpha) is a developmentally-regulated transcription factor and important regulator of gene expression during vertebrate development and carcinogenesis. The protein encoded by this gene is a member of the Kruppel-like zinc finger protein family and can repress expression of the AP-2 alpha gene by binding to a specific site in the AP-2 alpha gene promoter. Repression by the encoded protein requires binding with a corepressor, CtBP1. Two transcript variants encoding different isoforms have been found for this gene.
Background Reference 1:
Suda, S., (2006) Biochem. Biophys. Res. Commun. 344 (1), 246-252.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-462

List Size:
100 ul

List Price:

$519.00

Datasheet
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