Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

45-803 (1ug/ml) staining of nuclear HepG2 (A) and (0.5ug/ml) HeLa (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-803 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo
45-803 (1ug/ml) staining of nuclear HepG2 (A) and (0.5ug/ml) HeLa (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

KLF15 Antibody

CATALOG NUMBER: 45-803

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig
Immunogen:
The immunogen for this antibody is: VDHLLPVDENFSS-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in HeLa and in nuclear cell lysates of HepG2 (calculated MW of 44kDa according to NP_054798.1). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat, Mouse and Pig Kidney, and NIH3T3 for this particular batch.Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
KLF15
Additional Names:
KLF15, KKLF, Kruppel-like factor 15, KKLF protein, kidney-enriched Kruppel-like factor, DKFZp779M1320
Protein Accession Number:
NP_054798.1
Protein Gi Number:
7662494
Ncbi Gene Id Number:
28999
Background Reference 1:
Uchida S, Tanaka Y, Ito H, Saitoh-Ohara F, Inazawa J, Yokoyama KK, Sasaki S, Marumo F. Transcriptional regulation of the CLC-K1 promoter by myc-associated zinc finger protein and kidney-enriched Kruppel-like factor, a novel zinc finger repressor. Mol Cell Biol. 2000 Oct;20(19):7319-31.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-803

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369