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Western blot analysis of KLF17 in rat liver tissue lysate with KLF17 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of KLF17 in mouse liver tissue with KLG17 antibody at 5 μg/ml.
Western blot analysis of KLF17 in rat liver tissue lysate with KLF17 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

KLF17 Antibody

CATALOG NUMBER: 8041

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (81%), Chicken: (79%)
Immunogen:
KLF17 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human KLF17.
The immunogen is located within the last 50 amino acids of KLF17.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
KLF17 antibody can be used for detection of KLF17 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in rat samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Specificity:
KLF17 antibody is human, mouse and rat reactive. At least two isoforms of KLF17 are known to exist; this antibody will only detect the large isoform. KLF17 antibody is predicted to not cross-react with other KLF proteins.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Predicted Molecular Weight:
Predicted: 28, 39, 43 kDa
Observed: 38 kDa
Purification:
KLF17 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
KLF17 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
KLF17 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
KLF17
Additional Names:
Kruppel-like factor 17, zinc finger protein 393, ZNF393, Zfp393
Protein Accession Number:
NP_775755
Protein Gi Number:
104294874
Ncbi Gene Id Number:
128209
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KLF17, also known as ZNF393, is a member of the Sp/KLF family of transcription factors (1,2). KLF17 binds to the promoter and negatively regulates the transcription of Id-1, a key metastasis regulator in breast cancer (3). Suppression of KLF17 promotes breast cancer cell invasion and epithelial-mesenchymal transition (3). Down-regulated KLF17 expression is also associated tumor invasion and poor prognosis in hepatocellular carcinoma, suggesting that the level of KLF17 expression can serve as a prognostic indicator (4).
Background Reference 1:
Yan W, Burns KH, Ma L, et al. Identification of Zfp393, a germ cell-specific gene encoding a novel zinc finger protein. Mech. Dev. 2002; 118:233-9.
Background Reference 2:
van Vliet J, Crofts LA, Quinlan KG, et al. Human KLF17 is a new member of the Sp/KLF family of transcription factors. Genomics 2006; 87:474-82.
Background Reference 3:
Gumireddy K, Li A, Gimotty PA, et al. KLF17 is a negative regulator of epithelial-mesenchymal transition and metastasis in breast cancer. Nat. Cell Biol. 2009; 11:1297-304.
Background Reference 4:
Liu FY, Deng YL, Li Y, et al. Down-regulated KLF17 expression is associated with tumor invasion and poor prognosis in hepatocellular carcinoma. Med. Oncol. 2013; 30:425.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8041

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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