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45-805 staining (1ug/ml) of Human Heart lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
45-805 staining (1ug/ml) of Human Heart lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

KLF3 Antibody

CATALOG NUMBER: 45-805

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: LMFDPVPVKQEAMD-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa band observed in Human Heart lysates (predicted MW of 40kDa according to NP_057615.3). Recommended for use at 1-3ug/ml. Please note that the 40kDa Band is sometimes accompanied by a 80-90kDa band of unknown identity. Primary incubation was for 1 hour.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
KLF3
Additional Names:
KLF3, BKLF, Kruppel-like factor 3 (basic), basic kruppel like factor, MGC48279
Protein Accession Number:
NP_057615.3
Protein Gi Number:
156151371
Ncbi Gene Id Number:
51274
Background Reference 1:
Turner J, Crossley M. Basic Kruppel-like factor functions within a network of interactinghaematopoietic transcription factors. Int J Biochem Cell Biol. 1999 Oct;31(10):1169-74.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-805

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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