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Western blot analysis of lysates from Mouse colon using KLF3 Rabbit mAb at 1:13000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 45s.
Western blot analysis of lysates from Rat colon using KLF3 Rabbit mAb at 1:13000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5s.
Western blot analysis of lysates from Mouse colon using KLF3 Rabbit mAb at 1:13000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 45s.

KLF3 Rabbit Recombinant Antibody (83-593)

CATALOG NUMBER: 83-593

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:13000 - 1:52000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Mouse colon,Rat colon
Predicted Molecular Weight:
39kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Klf3
Additional Names:
Bklf; Tef-2; 9930027G08Rik
Protein Accession Number:
Q60980
Ncbi Gene Id Number:
16599
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enables RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Involved in negative regulation of transcription by RNA polymerase II. Predicted to be located in nucleoplasm. Is expressed in several structures, including alimentary system; brain; early conceptus; genitourinary system; and integumental system. Orthologous to human KLF3 (KLF transcription factor 3).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-593

List Size:
100 uL

List Price:

$477.00

Datasheet
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