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45-806 (0.1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-806 (0.1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

KLF4 Antibody

CATALOG NUMBER: 45-806

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: AVSDALLPSFST-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Experiments gave strong bands at 75-90kDa and a faint 50-kDa band in Human Duodenal lysate after 0.1ug/ml antibody staining. These bands correspond to earlier findings in literature (Piccinni et al, J. Biol. Chem., Vol. 279 (10), 9103-9114). All these bands were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.1-0.3ug/ml.
Positive Control 1:
Cat. No. 1364 - Human Duodenum Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
KLF4
Additional Names:
KLF4, GKLF, Kruppel-like factor 4 (gut), EZF, endothelial Kruppel-like zinc finger protein
Protein Accession Number:
NP_004226.2
Protein Gi Number:
194248077
Ncbi Gene Id Number:
9314
Background Reference 1:
Shields JM, Christy RJ, Yang VW. Identification and characterization of a gene encoding a gut-enriched Kruppel-like factor expressed during growth arrest. J Biol Chem. 1996 Aug 16;271(33):20009-17.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-806

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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