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Western blot analysis of KLHL15 in HeLa cell lysate with KLHL15 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of KLHL15 in HeLa cells with KLHL15 antibody at 5 μg/mL.
Immunofluorescence of KLHL15 in HeLa cells with KLHL15 antibody at 20 μg/mL.
Western blot analysis of KLHL15 in HeLa cell lysate with KLHL15 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

KLHL15 Antibody

CATALOG NUMBER: 5223

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
KLHL15 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human KLHL15.
The immunogen is located within the last 50 amino acids of KLHL15.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
KLHL15 antibody can be used for detection of KLHL15 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
KLHL15 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
KLHL15 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
KLHL15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KLHL15
Additional Names:
KLHL15 Antibody: HEL-S-305, KIAA1677, Kelch-like protein 15
Protein Accession Number:
NP_085127
Protein Gi Number:
226442729
Ncbi Gene Id Number:
80311
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KLHL15 Antibody: KLHL15 is a member of a growing superfamily of kelch repeat-containing proteins. The kelch motif was discovered in the sequence of the Drosophila kelch ORF1 protein and forms a conserved beta-propeller tertiary structure. These kelch-like proteins have been implicated in embryogenesis and carcinogenesis through cytoskeleton organization. KLHL15 mRNA is widely expressed in many tissues and is thought to be involved in protein ubiquination and cytoskeleton organization.
Background Reference 1:
Yoshida K. Identification and characterization of a novel kelch-like gene KLHL15 in silico. Oncol. Rep.2005; 13:1133-7.
Background Reference 2:
Robinson DN and Cooley L. Drosophila kelch is an oligomeric ring canal actin organizer. J. Cell Biol.1997; 138:799-810.
Background Reference 3:
Adams J, Kelso R, and Cooley L. The kelch repeat superfamily of proteins: propellers of cell function. Trends Cell Biol.2000; 10:17-24.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5223

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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