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45-808 (0.1ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-808 (3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-808 (0.1ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

KLK2 Antibody

CATALOG NUMBER: 45-808

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-KHQSLRPDEDSSHD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 22kDa band observed in Human Prostate lysates (calculated MW of 24.7kDa according to NP_001002231.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Prostate shows vesicular staining in the secretory cells. Recommended concentration: 2.5-3.8ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_005542.1; NP_001002231.1).
Positive Control 1:
Cat. No. 1312 - Human Prostate Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
KLK2
Additional Names:
KLK2, kallikrein-related peptidase 2, KLK2A2, MGC12201, hK2 , glandular kallikrein 2, kallikrein 2, prostatic
Protein Accession Number:
NP_005542.1,NP_001002231.1
Protein Gi Number:
5031829
Ncbi Gene Id Number:
3817
Background Reference 1:
Stephan C, Xu C, Brown DA, Breit SN, Michael A, Nakamura T, Diamandis EP, Meyer H, Cammann H, Jung K. Three new serum markers for prostate cancer detection within a percent free PSA-based artificial neural network. Prostate. 2006 May 1;66(6):651-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-808

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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