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Western blot analysis of KLRA1 in mouse spleen tissue lysate with KLRA1 antibody at (A) 1 μg/mL and (B) 2 μg/mL.
Immunocytochemistry of KLRA1 in 293 cells with KLRA1 antibody at 4 μg/mL.
Immunofluorescence of KLRA1 in 293 cells with KLRA1 antibody at 20 μg/mL.
Western blot analysis of KLRA1 in mouse spleen tissue lysate with KLRA1 antibody at (A) 1 μg/mL and (B) 2 μg/mL.
Trial Size

KLRA1 Antibody

CATALOG NUMBER: 4999

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
KLRA1 antibody was raised against a 13 amino acid synthetic peptide near the center of human KLRA1.
The immunogen is located within amino acids 140 - 190 of KLRA1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
KLRA1 antibody can be used for detection of KLRA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of KLRA1 are known to exist; this antibody recognizes only the long isoform.
Positive Control 1:
Cat. No. 1406 - Mouse Spleen Tissue Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Purification:
KLRA1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
KLRA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
KLRA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KLRA1P
Additional Names:
KLRA1 Antibody: Ly49, KLRA#, KLRA1, LY49L, Ly-49L
Protein Accession Number:
EAW96194
Protein Gi Number:
119616600
Ncbi Gene Id Number:
10748
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KLRA1 Antibody: KLRA1 (also known as Ly49L) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA1 is thought to be an activating receptor, inducing DAP12 phosphorylation in response to antibody-mediated cross-linking of KLRA1 on NK cells.
Background Reference 1:
Makrigiannis AP, Etzler J, Winkler-Pickett R, et al. Identification of the Ly49L protein: evidence for activating counterparts to inhibitory Ly49 proteins. J. Leuk. Biol.2000; 68:765-71.
Background Reference 2:
Smith HR, Karlhofer FM, and Yokoyama WM. Ly-49 multigene family expressed by IL-2-activated NK cells. J. Immunol.1994; 153:1068-79.
Background Reference 3:
Brennan J, Mager D, Jefferies W, et al. Expression of different members of the Ly-49 gene family defines distinct natural killer cell subsets and cell adhesion properties. J. Exp. Med.1994; 180:2287-95.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4999

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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