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Western blot analysis of KLRD1 Antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lymph with KLRD1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of CEM cells using KLRD1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of KLRD1 Antibody in K562 cell line lysates (35ug/lane)

KLRD1 Antibody

CATALOG NUMBER: 63-557

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This KLRD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-57 amino acids from the N-terminal region of human KLRD1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
21 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KLRD1
Additional Names:
Natural killer cells antigen CD94, KP43, Killer cell lectin-like receptor subfamily D member 1, NK cell receptor, CD94, KLRD1, CD94
Protein Accession Number:
Q13241
Protein Gi Number:
334302835
Ncbi Gene Id Number:
3824
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KLRD1 plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells.
Background Reference 1:
Chang,C., et.al., Eur. J. Immunol. 25 (9), 2433-2437 (1995)
Background Reference 2:
Rodriguez,A., et.al., Immunogenetics 47 (4), 305-309 (1998)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-557

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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