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45-079 staining (0.3ug/ml) of Human Spleen lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
45-079 staining (0.3ug/ml) of Human Spleen lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

KLRK1 Antibody

CATALOG NUMBER: 45-079

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-KVYSKEDQDLLK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in Human Lymph Node and Human Spleen lysates (calculated MW of 25.3kDa according to NP_031386.2). The observed molecular weight is explained by glycosylation (Han et al, Blood. 2004 Nov 1;104(9):2858-66. Epub 2004 Jul 6; PMID: 15238421). Recommended concentration: 0.1-0.3ug/ml. Primary incubated for 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Spleen, and Rat Thymus for this particular batch.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
KLRK1
Additional Names:
KLRK1, NKG2-D, killer cell lectin-like receptor subfamily K, member 1, HGNC:18788, D12S2489E, KLR, NK cell receptor D, NKG2-D type II integral membrane protein, CD314, D12S2489E, FLJ17759, FLJ75772, NKG2D
Protein Accession Number:
NP_031386.2
Protein Gi Number:
6679052
Ncbi Gene Id Number:
22914
Background Reference 1:
Hayakawa Y, Smyth MJ. Innate immune recognition and suppression of tumors. Adv Cancer Res. 2006;95:293-322

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-079

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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