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Western blot analysis of extracts of various cell lines, using KMT5C antibody (16-505) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Western blot analysis of extracts of various cell lines, using KMT5C antibody (16-505) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

KMT5C Antibody

CATALOG NUMBER: 16-505

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human KMT5C (NP_116090.2).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
293T
Positive Control 2:
HeLa
Positive Control 3:
K-562
Positive Control 4:
SH-SY5Y
Positive Control 5:
Mouse brain
Predicted Molecular Weight:
Observed: 52kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
KMT5C
Additional Names:
SUV420H2, Suv4-20h2, histone-lysine N-methyltransferase KMT5C, histone-lysine N-methyltransferase SUV420H2, lysine (K)-specific methyltransferase 5C, lysine N-methyltransferase 5C, lysine-specific methyltransferase 5C, su(var)4-20 homolog 2, suppressor of variegation 4-20 homolog 2
Ncbi Gene Id Number:
84787
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SUV420H2 and the related enzyme SUV420H1 (MIM 610881) function as histone methyltransferases that specifically trimethylate nucleosomal histone H4 (see MIM 602822) on lysine-20 (K20).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-505

List Size:
100 uL

List Price:

$540.00

Datasheet
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