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Western blot analysis of KNG1 using rabbit polyclonal KNG1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KNG1 gene.
Western blot analysis in Ramos cell line lysates (35ug/lane).
KNG1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of KNG1 using rabbit polyclonal KNG1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KNG1 gene.

KNG1 Antibody

CATALOG NUMBER: 55-985

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This KNG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-166 amino acids from the N-terminal region of human KNG1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
72 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KNG1
Additional Names:
Kininogen-1, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, High molecular weight kininogen, HMWK, Williams-Fitzgerald-Flaujeac factor, Kininogen-1 heavy chain, T-kinin, Ile-Ser-Bradykinin, Bradykinin, Kallidin I, Lysyl-bradykinin, Kallidin II, Kininogen-1 light chain, Low molecular weight growth-promoting factor, KNG1, BDK, KNG
Protein Accession Number:
P01042
Protein Gi Number:
124056474
Ncbi Gene Id Number:
3827
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene uses alternative splicing to generate twodifferent proteins- high molecular weight kininogen (HMWK) and lowmolecular weight kininogen (LMWK). HMWK is essential for bloodcoagulation and assembly of the kallikrein-kinin system. Also,bradykinin, a peptide causing numerous physiological effects, isreleased from HMWK. In contrast to HMWK, LMWK is not involved inblood coagulation. Three transcript variants encoding differentisoforms have been found for this gene.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 3:
Houlihan, L.M., et al. Am. J. Hum. Genet. 86(4):626-631(2010)
Background Reference 4:
Khan, M.M., et al. Am. J. Physiol. Heart Circ. Physiol. 298 (2), H652-H658 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-985

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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