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Western blot analysis of various lysates using CDK9 Rabbit mAb (A11145) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 3min.
Western blot analysis of lysates from wild type(WT) and CDK9 knockout (KO) 293T cells, using [KO Validated] CDK9 Rabbit mAb (A11145) at 1:500 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] CDK9 Rabbit mAb (A11145) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KO Validated] CDK9 Rabbit mAb (A11145) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KO Validated] CDK9 Rabbit mAb (A11145) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using [KO Validated] CDK9 Rabbit mAb (A11145) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using [KO Validated] CDK9 Rabbit mAb (A11145) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KO Validated] CDK9 Rabbit mAb (A11145) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Confocal imaging of U-2 OS cells using [KO Validated] CDK9 Rabbit mAb (A11145,dilution 1:50)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg CDK9 antibody (A11145). Western blot was performed from the immunoprecipitate using CDK9 antibody (A11145) at a dilution of 1:1000.
Western blot analysis of various lysates using CDK9 Rabbit mAb (A11145) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 3min.

[KO Validated] CDK9 Rabbit Recombinant Antibody

CATALOG NUMBER: 81-116

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, IHC-P, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, IHC-P, IP, WB
Application Note:
WB,1:500 - 1:3000, IHC-P,1:200 - 1:2000, IF/ICC,1:50 - 1:400, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
HeLa, 293T ,MCF7, Mouse brain, Rat testis
Predicted Molecular Weight:
43kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CDK9
Additional Names:
TAK; C-2k; CTK1; CDC2L4; PITALRE; K9
Protein Accession Number:
P50750
Ncbi Gene Id Number:
1025
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

81-116

List Size:
100 uL

List Price:

$540.00

Datasheet
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