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Western blot analysis of lysates from wild type (WT) and N-terminal alpha-synuclein knockout (KO) HIPP Neurons cells using [KO Validated] N-terminal alpha-synuclein mAb [MJFF-B-3A4] (A27117) at 1:5000 dilution . | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 10s.
Western blot analysis of various lysates using [KO Validated] N-terminal alpha-synuclein mAb [MJFF-B-3A4] (A27117) at 1:5000 dilution . | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Negative control (NC): T-47D | Exposure time: 60s.
Western blot analysis of various lysates using [KO Validated] N-terminal alpha-synuclein mAb [MJFF-B-3A4] (A27117) at 1:5000 dilution . | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 3s.
Confocal imaging of Mouse primary hippocampal neuron cells(Wild type) and Mouse primary hippocampal neuron cells(SNCA KO) using N-terminal alpha-synuclein mAb [MJFF-B-3A4] (A27117, dilution 1:500) followed by a further incubation with AF488 conjugated donkey Anti-Rabbit (H+L) (A-21206, dilution 1:500) (Green). The cells were counterstained with total aSyn antibody and MAP2 antibody followed by incubation with AF647 conjugated Donkey Anti-mouse IgG (H+L) Ab (A-31571) (Red) and AF568 conjugated Donkey Anti-chicken IgG (H+L) Ab (703-545-155, dilution 1:400) (Grey).DAPI was used for nuclear staining (Blue). Objective: 40x.
Western blot analysis of lysates from wild type (WT) and N-terminal alpha-synuclein knockout (KO) HIPP Neurons cells using [KO Validated] N-terminal alpha-synuclein mAb [MJFF-B-3A4] (A27117) at 1:5000 dilution . | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 10s.

[KO Validated] N-terminal alpha-synuclein Recombinant Antibody [MJFF-B-3A4]

CATALOG NUMBER: 83-469

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, WB
Application Note:
WB,1:5000 - 1:30000, IF/ICC,1:200 - 1:500, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
14kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SNCA
Additional Names:
NACP; PARK1; α-Synuclein; SNCA
Protein Accession Number:
P37840
Ncbi Gene Id Number:
6622
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Alpha-synuclein is a neuronal protein that plays many roles in synaptic activity such as membrane trafficking of synaptic vesicles and neurotransmitter release. Multiplications in SNCA have been implicated in genetic forms of Parkinson disease and pathological post-translational modifications of alpha-synuclein are a hallmark of Parkinson’s disease. These modifications include phosphorylation, nitration, acetylation, truncation, and aggregation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-469

List Size:
100 uL

List Price:

$510.00

Datasheet
Shipping Info

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