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Western blot analysis of lysates from Ythdc1-cKO mouse ES cells, using [KO Validated] Ythdc1 Rabbit mAb (A25682) at 1:1000 dilution incubated overnight at 4℃. Ythdc1-cKO mouse ES cells were treated by 4OHT. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 45s.
Western blot analysis of lysates from HeLa cells using [KO Validated] Ythdc1 Rabbit mAb (A25682) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 45s.
Western blot analysis of lysates from Ythdc1-cKO mouse ES cells, using [KO Validated] Ythdc1 Rabbit mAb (A25682) at 1:1000 dilution incubated overnight at 4℃. Ythdc1-cKO mouse ES cells were treated by 4OHT. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 45s.

[KO Validated] Ythdc1 Rabbit Recombinant Antibody

CATALOG NUMBER: 83-136

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:1000 - 1:4000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Mouse ES cells, HeLa
Predicted Molecular Weight:
85kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Ythdc1
Additional Names:
mKIAA1966; A730098D12Rik; Mouse Ythdc1
Protein Accession Number:
E9Q5K9
Ncbi Gene Id Number:
231386
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Predicted to enable N6-methyladenosine-containing RNA binding activity and mRNA binding activity. Involved in posttranscriptional regulation of gene expression. Acts upstream of or within gamete generation; in utero embryonic development; and regulation of mRNA processing. Located in nucleus. Orthologous to human YTHDC1 (YTH domain containing 1).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-136

List Size:
100 uL

List Price:

$540.00

Datasheet
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