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45-812 staining (0.03ug/ml) of HeLa lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
45-812 staining (top panel and green) of 4% formaldahyde-fixed HeLa with endogenously expressed HistonH2B-GFP fusion as DNA marker (mid panel and red). Data kindly provided by Francisco Iborra, MRC Mol. Haematol. Unit, Oxford. <strong>This data is from a
45-812 staining (0.03ug/ml) of HeLa lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

KPNA2 Antibody

CATALOG NUMBER: 45-812

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-QVQDGAPGTFNF
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in lysates of cell line HeLa (calculated MW of 57.9kDa according to NP_002257.1). Recommended for use at 0.03-0.1ug/ml. Primary incubation was 1 hour.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
KPNA2
Additional Names:
KPNA2, IPOA1 QIP2, RCH1, SRP1alpha, karyopherin alpha 2 (RAG cohort 1, importin alpha 1), RAG cohort 1, importin alpha 1, importin alpha 2, importin-alpha-P1, karyopherin alpha 2, pendulin
Protein Accession Number:
NP_002257.1
Protein Gi Number:
4504897
Ncbi Gene Id Number:
3838
Background Reference 1:
Cuomo CA, Kirch SA, Gyuris J, Brent R, Oettinger MA. Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein. Proc Natl Acad Sci USA. 1994 Jun 21;91(13):6156-60.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-812

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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