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Western blot analysis of KPNA2 in rat heart tissue lysate with KPNA2 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of KPNA2 in human heart tissue with KPNA2 antibody at 5 μg/mL.
Immunofluorescence of KPNA2 in human heart tissue with KPNA2 antibody at 20 μg/mL.
Western blot analysis of KPNA2 expression in Daudi cell lysate with KPNA2 antibody at (A) 1 and (B) 2 μg/ml.
Western blot analysis of KPNA2 in rat heart tissue lysate with KPNA2 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

KPNA2 Antibody

CATALOG NUMBER: 5983

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
KPNA2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human KPNA2.
The immunogen is located within amino acids 40 - 90 of KPNA2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
KPNA2 antibody can be used for detection of KPNA2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1461 - Rat Heart Tissue Lysate
Positive Control 2:
Cat. No. 10-501 - Human Heart Tissue Slide
Positive Control 3:
Cat. No. 1224 – Daudi Cell Lysate
Purification:
KPNA2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
KPNA2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
KPNA2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KPNA2
Additional Names:
KPNA2 Antibody: QIP2, RCH1, IPOA1, SRP1alpha, SRP1, Importin subunit alpha-1, Karyopherin subunit alpha-2
Protein Accession Number:
NP_002257
Protein Gi Number:
4504897
Ncbi Gene Id Number:
3838
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KPNA2 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA2 is the alpha subunit 2 of karyopherin, which forms a complex with importin subunit beta-1 and functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. It is ubiquitously expressed and contains an IBB/importin beta domain, ten Armadillo repeats that bind "cargo" and three intervening nuclear localization sequences (NLSs). It has recently been reported to play an important role in tumorigenesis and tumor progression.
Background Reference 1:
Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72.
Background Reference 2:
Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem.2003; 278: 51937-49.
Background Reference 3:
Tseng SF, Chang CY, Wu KJ, et al. Importin KPNA2 is required for proper nuclear localization and multiple functions of NBS1. J. Biol. Chem.2005; 280:39594-600.
Background Reference 4:
Sakai M, Sohda M, Miyazaki T, et al. Significance of karyopherin-{alpha} 2 (KPNA2) expression in esophageal squamous cell carcinoma. Anticancer Res.2010; 30:851-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5983

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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