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45-813 (0.1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-813 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.<strong>This data is from a previous batch, not on sale.</strong>
45-813 (0.1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

KPNA3 Antibody

CATALOG NUMBER: 45-813

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-DPTANLQTKEFNF
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in lysates of cell line HeLa (calculated MW of 57.8kDa according to NP_002258.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
KPNA3
Additional Names:
KPNA3, IPOA4, karyopherin alpha 3 (importin alpha 4), RP11-432M24.3, SRP1gamma, SRP4, hSRP1, importin alpha 4, importin alpha 4, importin-alpha-Q2, karyopherin alpha 3, SRP1, importin alpha-3
Protein Accession Number:
NP_002258.2
Protein Gi Number:
34485722
Ncbi Gene Id Number:
3839
Background Reference 1:
Kohler M, Ansieau S, Prehn S, Leutz A, Haller H, Hartmann E. Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family. FEBS Lett. 1997 Nov 3;417(1):104-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-813

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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