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Western blot analysis of KPNA3 in EL4 cell lysate with KPNA3 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of KPNA3 in HeLa cells with KPNA3 antibody at 10 μg/mL.
Immunofluorescence of KPNA3 in EL4 cells with KPNA3 antibody at 20 μg/mL.
Western blot analysis of KPNA3 in EL4 cell lysate with KPNA3 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

KPNA3 Antibody

CATALOG NUMBER: 5985

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
KPNA3 antibody was raised against a 14 amino acid synthetic peptide near the carboxy end of human KPNA3.
The immunogen is located within the last 50 amino acids of KPNA3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
KPNA3 antibody can be used for detection of KPNA3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Positive Control 3:
Cat. No. 17-207 - EL4 Cell Slide
Purification:
KPNA3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
KPNA3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
KPNA3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KPNA3
Additional Names:
KPNA3 Antibody: SRP1, SRP4, IPOA4, hSRP1, SRP1gamma, QIP2, Importin subunit alpha-4, Importin alpha Q2, Qip2
Protein Accession Number:
NP_002258
Protein Gi Number:
34485722
Ncbi Gene Id Number:
3839
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KPNA3 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA3, the alpha subunit 3 of karyopherin, is silimar to human KPNA2, suggesting that KPNA3 may be involved in the nuclear transport system. It is ubiquitously expressed and recent studies have shown that human KPNA3 may be associated with schizophrenia.
Background Reference 1:
Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72.
Background Reference 2:
Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem.2003; 278: 51937-49.
Background Reference 3:
Takeda S, Fujiwara T, Shimizu F, et al. Isolation and mapping of karyopherin alpha 3 (KPNA3), a human gene that is highly homologous to genes encoding Xenopus importin, yeast SRP1 and human RCH1. Cytogenet. Cell Genet.1997; 76:87-93.
Background Reference 4:
Zhang H, Ju G, Wei J, et al. A combined effect of the KPNA3 and KPNB3 genes on susceptibility to schizophrenia. Neurosci. Lett.2006; 402:173-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5985

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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