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45-814 (1ug/ml) staining of Human Testes (A), Human Ovary (B), Mouse Testes (C), Rat Testes (D) and Rat Ovary (E) lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.
45-814 (3ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-814 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-814 (1ug/ml) staining of Human Testes (A), Human Ovary (B), Mouse Testes (C), Rat Testes (D) and Rat Ovary (E) lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

KPNA4 Antibody

CATALOG NUMBER: 45-814

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-NSSANVPTEGFQF
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Testis and Ovary and approx. 60kDa in Rat Testis and Ovary and Mouse Testis lysates (calculated MW of 57.9kDa according to Human NP_002259.1, Mouse NP-032493.1 and Rat NP_001014793.1). Recommended concentration: 0.5-1ug/ml. Primary incubation 1 hour at room temperature.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
KPNA4
Additional Names:
KPNA4, IPOA3, QIP1, SRP3, MGC12217, MGC26703, karyopherin alpha 4 (importin alpha 3), importin alpha 3, importin-alpha-Q1, karyopherin alpha 4
Protein Accession Number:
NP_002259.1
Protein Gi Number:
4504901
Ncbi Gene Id Number:
3840
Background Reference 1:
Seki T, Tada S, Katada T, Enomoto T. Cloning of a cDNA encoding a novel importin-alpha homologue, Qip1: discrimination of Qip1 and Rch1 from hSrp1 by their ability to interact with DNA helicase Q1/RecQL. Biochem Biophys Res Commun. 1997 May 8;234(1):48-53.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-814

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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