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Western blot analysis of KPNA4 in HeLa cell lysate with KPNA4 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunocytochemistry of KPNA4 in HeLa cells with KPNA4 antibody at 2.5 μg/mL.
Immunofluorescence of KPNA4 in HeLa cells with KPNA4 antibody at 20 μg/mL.
Western blot analysis of KPNA4 in HeLa cell lysate with KPNA4 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

KPNA4 Antibody

CATALOG NUMBER: 5987

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
KPNA4 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human KPNA4.
The immunogen is located within amino acids 30 - 80 of KPNA4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
KPNA4 antibody can be used for detection of KPNA4 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
KPNA4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
KPNA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
KPNA4 antibody can be stored at 4°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KPNA4
Additional Names:
KPNA4 Antibody: QIP1, SRP3, IPOA3, QIP1, Importin subunit alpha-3, Importin alpha Q1, Qip1
Protein Accession Number:
EAW78632
Protein Gi Number:
119599038
Ncbi Gene Id Number:
3840
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KPNA4 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. Molecules containing the classical nuclear localization signal (NLS) are transported into the nucleus by alpha/beta heterodimers. KPNA3 has been shown to be important in the TNF-α-induced nuclear import of NF-κB. It is also involved in the stress-mediated nuclear stabilization of p53 and the nuclear import and replication of HIV-1 in both dividing and non-dividing cells.
Background Reference 1:
Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72.
Background Reference 2:
Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem.2003; 278: 51937-49.
Background Reference 3:
Goldfarb DS, Corbett AH, Mason DA, et al. Importin alpha: a multi-purpose nuclear-transport receptor. Trends Cell Biol.2004; 14:505-14.
Background Reference 4:
Fagerlund R, Melen K, Cao X, et al. NF-kappaB p52, RelB, and c-Rel are transported into the nucleus via a subset of importin alpha molecules. Cell Signal.2008; 20:1442-51.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5987

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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